Thermal Cyclers - Techne
 

48-Well Thermal Cyclers

TC-3000G

The world's smallest gradient cycler, the TC-3000G will hold half a 96-well plate, in a horizontal format so that it offers 8 columns for annealing temperature optimisation and 6 rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over a 15°C gradient between the temperatures of 20 and 80°C.

All the features of the TC-3000 and TC-3000X plus...

•Gradient block: A gradient of up to 15°C between the temperatures of 20 and 80°C can be set, allowing any protocol to be optimised within a single experiment.
•New software: Enables a gradient to be added to any temperature step of the program.
•Gradient calculator: The gradient calculator function displays the temperature for each of the 8 columns, ensuring easy replication of thermal conditions.

Full Size 96-well Thermal Cyclers

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and ideal for a larger number of parallel samples. Both units offer:

•Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
•Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
•Temperature range of 4°C to 99°C
•8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
•Incremental/decremental time and temperature for applications such as Touchdown PCR
•The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software

TC-5000

The TC-5000 gradient thermal cycler enables you to optimise your experiments at the `touch of a screen', with the same mechanical features as the TC-4000.

•Real-time' graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment's status.
•User-friendly programming interface provided by a 115 x 90mm touch-sensitive screen.
•Excellent heating rate: A maximum heating rate of 3°C/sec and block uniformity of ±0.3°C at 50°C with or without a gradient...no compromise!
•The most linear gradient cycler on the market, with an amazing range of 30°C. 
•The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.
•Gradient range 20°C to 70°C, maximum 30°C
•Decremental/incremental time and temperature

TC-Plus Thermal Cyclers

The TC-PLUS thermal cyclers have all the latest features from a colour touchscreen to fast ramp rates along with some innovative additions such as an automatic, non-motorised heated lid, TERS®, thermal energy recovery system and a unique space-saving, stackable design. The TC-PLUS and Satellite units can be combined to form any multi-block format with the Satellites being controllable from either a PC or the TC-PLUS.

Key Features

•Colour touchscreen for fast program setup.
•TERS®, Thermal Energy Recovery System to save on energy consumption.
•Unique, easy to use CD-type sample drawer mechanism which provides the correct pressure for use with caps: domed or flat, heat OR adhesive seals without the use of a motor!
•Space-saving, stackable design to save on bench space!
•Satellite option which can be controlled by either the TC-PLUS or a PC using Techne Workbench software.
•Fast ramp rate of up to 5°C/sec.
•Three gradient block options; 96 x 0.2ml, 60 x 0.5ml and 384-well.
•Tm calculator, which uses the Nearest Neighbour algorithm.
•Backed by Techne's 4 year warranty.*

*4 year for the TC-PLUS thermal cycler and 80,000 cycles or 4 year warranty for the thermal block, whichever comes first.

Technical Information

Thermal Cyclers - Techne
 

Full Size 96-well Thermal Cyclers

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and ideal for a larger number of parallel samples. Both units offer:

• Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
• Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
• Temperature range of 4°C to 99°C
• 8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
• Incremental/decremental time and temperature for applications such as Touchdown PCR
• The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software

TC-4000

The TC-4000 is one of the most affordable full sized thermal cyclers in the market, flexible for all your protocols and easy on your budget.

•Intuitive programming: 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.
•Excellent heating rate of 2.6°C/sec and block uniformity of ±0.3°C at 50°C, ensuring optimal reproducibility
 

Technical Information

Thermal Cyclers - Techne

48-Well Thermal Cyclers

TC-3000X

The new TC-3000X personal cycler is based on the unrivalled and reliable TC-3000. The new model is ideal for those laboratories that need just that little bit more, with its expanded sample capacity to accommodate 48 x 0.2ml tubes or for convenience, a 48-well plate.

• Increased sample capacity: The footprint remains the same, only 561cm2,but the capacity increases to 48 x 0.2ml or 30 x 0.5ml tubes
• Adjustable heated lid: The heated lid can now be adjusted for use with all PCR tubes, strip tubes or 48-well plates sealed with sealing mats
• Rapid heating rate: Utilising the latest Peltier technology the TC-3000X has a heating ramp rate of 3.3°C/sec and a 2.0°C/sec cooling rate
• Fast-track programming: Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple
• PC control & networking: The TC-3000X can be linked together with other Techne cyclers; up to 32 cyclers can be networked to one computer
 

Technical Information

Thermal Cyclers - Techne
 

TC-3000 Personal 25-well Thermal Cycler

The TC-3000 has been designed with research and teaching laboratories in mind, the TC-3000 offers the ultimate in low cost solutions where ease of use is high on the priority list. Experience in thermal cycler manufacturing since 1987, ensures that the TC-3000 provides guaranteed performance and quality results, every time.

•Space saving small footprint: at only 561cm2, the TC-3000 is even economical on space
•Rapid heating rate with a 3.6°C/sec heating rate and 2.0°C/sec cooling rate 
•Adjustable heated lid (100°C to 115°C), designed to reduce the risk of sample evaporation, can be enabled or disabled. The heated lid only comes on if the block is set above 35°C 
•Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple 
•The TC-3000 can be linked together with up to 32 cyclers can be networked to one computer
 

Technical Information

SPECIFICATION

BLOCK TEMPERATURE

*specifications given are typical values in ambient temperature of 20 ºC for non-gradient (unless stated).
#Actual numbers dependent on complexity of the programs. 

Progene Thermal Cycler

Smallest in the World*

• Fastest in the World*
• Least expensive in the world*
• Nothing is extra - an amazing list of innovative features as standard and with "user friendly" software that can accommodate all the latest PCR protocols such as "Touchdown", "RAPD", and "Long PCR".
• Unrivaled performance - better than 2.2°C cooling 2.8°C heating per second in the 55°C to 90°C range - dependent on block type.
• Temperature range 4°C to 99°C
• Heated lid for oil free PCR
• Two models available for 25 x 0.2ml micro tubes/ 5x5 multiwell plate and 20 x 0.5ml microcentrifuge tubes
• Full two year warranty

GENIUS Thermal Cycler Specifications

GENIUS Thermal Cycler Computer Connectivity Features

GeniusQuad Package
A reduced price package containing all you require for high throughput cycling with the option to connect through a PC:

o 4 x Genius cyclers in 96 or 384 microtitre plate formats
o 1 x Genius control software
o 1 x Computer connector cable with RS232: RS458 converter, to the first cycler to a PC
o 4 x Genius connector boxes and connection cables to PC-enabled each cycler
o 4 x Genius cyclers in 96 or 384 microtitre plate formats
o Safe: No exposure to boiling agarose and reduced handling of carcinogenic Ethidium Bromide.

Genius Thermal Cycler

Regardless of the block type being used all samples are heated to a programmed temperature and held to very precise limits. 

Speed - Throughput of samples is quick - due to overall performance of the heating and cooling cycle of the Geniusa 30 cycle program can be completed in well under one hour, freeing the instrument for several PCR runs in a normal working day. 

Performance - In addition to the speed of heating and cooling the on board processor PRT senior ensure a high degree of temperature stability and uniformity of the block so that all samples are processed to the programmed requirements. 

The Genius Thermal Cycler comes complete with a heated lid as standard and is designed to reduce to a minimum refluxing of the sample onto the lid and side walls of the sample tube. 

Flexigene/Flexigene Quad Thermal Cyclers

The Flexigene is a high performance, high sample throughput instrument at a highly affordable price.

Flexible block format: The truly user-friendly fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need of any tools (60 x 0.5ml, 96 x 0.2ml, 96 x 0.2ml for fully skirted plates, 384-well or flat plate for in-situ).

New design flexible heated lid: Defined pressure, programmable heated lid to accommodate a variety of consumables.

Intuitive programming: New 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.

8 Peltiers: Building on the innovative Quad Circuit Technology of the Touchgene Gradient, the Flexigene ensures unsurpassed control accuracy and temperature uniformity.

Low cost: The Flexigene is probably the most affordable full sized Thermal Cycler.

PC networking: The Flexigene can be connected to a PC along with other Techne cyclers for low volume or high throughput applications using Gensoft*.

Specifications

Blocks Formats

Block Temperature Gradient

Net weight

11kg (total)

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The new Flexigene thermal cycler, flexible on your protocols, easy on your budget.

The Flexigene is a high performance, high sample throughput instrument at a highly affordable price.

Flexible block format: The truly user-friendly fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need of any tools (40 x 0.5ml, 96 x 0.2ml, 96 x 0.2ml for fully skirted plates, 384-well or flat plate for in-situ).

New design flexible heated lid: Defined pressure, programmable heated lid to accommodate a variety of consumables.

Intuitive programming: New 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.

8 Peltiers: Building on the innovative Quad Circuit Technology of the Touchgene Gradient, the Flexigene ensures unsurpassed control accuracy and temperature uniformity.

Low cost: The Flexigene is probably the most affordable full sized Thermal Cycler.

* Up to 32 Techne cyclers can be connected and run through a PC using Gensoft,
a software package which can be downloaded free of charge from their website.

FlexiQuad Package

Unbeatable value

This reduced price package containing all you require for high throughput cycling with the option to connect through a PC:

• 4 x Flexigene cyclers in 96 standard, 96 fully skirted or 384 well microtitre plate format.
• Gensoft control software (also free of charge from the our website).
• 1 x computer connector cable with RS232:RS485 converter, to connect the first cycler to a PC
• 4 x Flexigene connector boxes and connections.
• 1 x termination box with power pack and power cable, to place on the last cycler.

Specifications

Blocks Formats

Block Temperature Gradient

Net weight

* Given are typical values of a standard 0.2ml block, in an ambient temperature of 20°C.
# Actual numbers are dependent on complexity of the programs. All Techne cyclers have a full 2 year Warranty.

o 4-Fold control: Each block consists of 8 peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block. This ensures that the TC-512 exhibits the most accurately controlled linear gradient available*.
 
o High performance: A maximum heating rate of 3°C/Sec and block uniformity of ±0.4°C. With or without a gradient......no compromise!
 
o Versatile block format: The truly user-friendly interchangeable block system allows blocks to be interchanged in a matter of seconds without the need of any tools (60 x 0.5ml 96 x 0.2 ml, 3841 well, Insitu Block2).
 
o Proven track record: Having more than 13 years experience in thermal cycler design ensures that the Techne TC-512 encompasses reliability with all the specifications you could wish. These include: PC control and networking, decremental/incremental time and temperature, and defined pressure programmable heated lid.
 
o Intuitive programming: Easy to use software with free upgrades downloadable from the Techne website as new functionality is added.
 
o New Design Flexible Heated Lid: Defined pressure, high quality, programmable heated lid to accommodate a variety of consumables..
 

SPECIFICATION
 

BLOCK TEMPERATURE

*specifications given are typical values in ambient temperature of 20 ºC for non-gradient (unless stated).
#Actual numbers dependent on complexity of the programs. 

Ovens - Hybridisation - Techne
 

Hybrigene (HB-3D)

• Temperature range from 10°C above ambient to 80°C
• Can hold up to 16 mini or 4 large glass tubes
• Flexibility at an affordable price, offering excellent temperature accuracy and uniformity
• The Hybrigene is a compact, stackable alternative to the Hybridiser HB-1D - for when space is important
• Stack up to 3 ovens, saving on valuable laboratory space

HB-1D

• The famous Hybridiser HB-1D is compact, easy to use and provides complete protection from hybridisation hazards
• A temperature range of 10°C above ambient to 100°C
• High capacity which can hold up to 24 mini tubes or 6 unique large tubes
• Hybridisations can be performed with a minimal volume of 5ml of probe
• Adjustable feet to enable accurate leveling
• Unique double-glazed glass door; quiet and safe, providing durable protection

Technical Information

• 3 sizes available:
  small & mini and unique
  large tubes

• Durable:
  thick borosilicate glass

• Easy pour:
  non drip, safe and convenient

• Sealing Ring:
  “O” ring sealed end caps
  allow no leakage!

• Broken thread on screw cap:
  easy opening with no vacuum problems

• Tube Assemblies:
  Adaptors to hold multiple
  small glass tubes to
  increase capacity.

Tube holders
Tube holders with clips, available to carry 16 x 15ml or
8 x 50 ml tubes. Ideal for a higher throughput of smaller samples.

Adjustable speed rocking platform
Fits on the base of the unit still allowing hybridisation tubes to be placed above. Enables membranes to be processed using a wave motion. Available in fast or slow speeds, which range from 5 to 60 oscillations per minute.

Static shelf 
Up to 2 (Hybrigene) or 3 (Hybridiser HB-1D) static stainless steel mesh shelves can be placed into the tube locators allowing the unit to be used as a simple non-motion incubator. Each shelf holds up to 4 kg.

Membrane separators 
Strong, re-usable porous membranes for placing between blots. Ensure even hybridisation when processing multiple hybridisation membranes in one tube. Available as packs of 5 (20x20 cm).

Hybridisation glass tube holder
Useful storage facility for tubes during membrane loading or when not in use. Carries up to 3 large and 3 small tubes.

16 x 15 ml tube holder

Rocking platform

Static shelf 

Hybridisation glass tube holder

Application notes:

Hybridisation protocol tips 
Hybridisation techniques which involve the detection of membrane bound molecules are commonplace in most laboratories and include Northern (RNA), Southern (DNA) and Western (Protein) blot. These may come in the form of molecules transferred from a gel, slot or dot blots (where they are spatially placed on top of the membrane using a pipette or specialised manifold), or by colony/plaque adhesion. During the hybridisation process, while larger volumes of wash buffers are used, small volumes of probe and detection solutions are desired, all requiring a variety of temperatures. Below are some useful tips which improve the quality of your results.

Hybridisation vessels 
To optimise your results use specially designed hybridisation incubators with rotating bottles. These offer many advantages including: reduced reagent volume, even membrane hybridisation, multiple membranes/vessel, easy probe recovery, temperature accuracy and reproducibility, safe liquid transfer.

Multiple membranes 
When a large number of blots are to be hybridised with the same probe, time and reagents can be saved by placing them together. To do this, pre-warm the first reagent and place it in the pre-warmed incubation vessel before adding the membranes one by one, always ensuring that each one is fully coated before adding the next. To optimise probe: target accessibility use separation membranes between each blot.

Improving signal: noise ratios
One of the most difficult aspects of membrane hybridisation is to keep a clean particulate free blot with a low background, while still retaining a good signal:

• Temperature: Chose the correct temperature. The easiest way to decrease the background during RNA and DNA blot hybridisation procedures is to increase the temperature at which the probe and initial post-hybridisation steps are carried out. As a guide, for a longer DNA probe (e.g. 200bp) start with 65°C and for an oligonucleotide probe try 50°C. For protein detection, some primary antibodies are more specific when hybridised overnight at 4°C rather than a few hours at room temperature.

• Probes & antibodies: Take care to keep stocks of probes and detection reagents at the recommended temperatures. For non-radioactive techniques, re-using probe and antibody hybridisation solutions can lead to significant improvements of signal (store antibodies at 4°C and DNA at -20°C). When using antibodies, give the stock tubes a quick spin in a microcentrifuge and pipette from the top of the liquid to remove background causing precipitants.

• Clean equipment: Cleanliness plays an important part in membrane hybridisations. Always wear gloves, handle blots with forceps and avoid dusty environments. For non-radioactive detection techniques, microwave the reaction vessels with some water in prior to re-use as this acts to remove residues which can cause serious background problems.

• Buffers: Only use high quality reagents and ultra-pure water. When making up stocks of large volume buffers such as SSC, pass them through 0.45 µm filters to remove particulates. If using formamide, make sure it has been deionized first.

• Crosslinking: Using a UV-Crosslinker  to irreversibly bind your nucleic acids to the membrane is faster and much more efficient than traditional baking and results in a higher signal.
   

DB-4D ambient to 100°C, requires 4 lnsert blocks

Block heater Techne dry requires 3 blocks

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

• Very accurate temperature control
• Analogue or digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 

DB-3

• The DB-3 is designed to hold up to 3 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Temperature range from ambient to 100°C
• Fast heat-up rate: 30°C to 100°C in just 18 minutes
• Temperature stability at 40°C: ± 0.05°C

DB-3A

• Temperature range from ambient to 200°C for higher temperature applications
• Can hold up to 3 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Powerful heater for fast heat-up rate: 30°C to 200°C in just 30 minutes
• Temperature stability at 40°C: ±0.1°C
 

DB-3D and DB-3DL

• Can hold up to 3 aluminium insert blocks or one 96-well plate block
• Bright orange LED digital display for fast and accurate setting of temperature
• DB-3DL temperature range from ambient to 100°C and DB-3D up to 200°C
• Powerful heater for fast heat-up rate: 30°C to 200°C in just 25 minutes
• Interchangeable insert blocks to accommodate a variety of tubes
• Temperature stability at 40°C: ± 0.1°C

Technical Specification
 

Ordering Information
 

Block inserts need to be purchased separately

Block heater Techne dry requires 2 blocks

Techne's Dri-block® heaters provide a safe, dry, constant temperature source in the laboratory. The units are particularly suitable for microbiology and clinical laboratories for incubation, boiling, inactivation, wet ashing, sample concentration, enzyme analysis and many other clinical and industrial purposes.

• Very accurate temperature control
• Analogue or digital control
• Wide range of interchangeable aluminium blocks
• Blocks available as accessories for all applications - tubes, vials and microplates
• Block extraction tool is supplied, allowing blocks to be removed easily
• 3-year warranty as standard
 

DB-2A

• Small, light and compact footprint, economical price
• Can hold up to 2 aluminium insert blocks or one 96-well plate block
• Analogue: temperature setting is by a calibrated dial
• Temperature range from ambient to 100°C
• Fast heat-up rate: 30°C to 100°C in just 12 minutes
• Temperature stability at 40°C: ± 0.05°C

DB-2D and DB-2DH

• Bright orange LED digital display for fast and accurate setting of temperature
• RS232 connection available for PC control
• Can hold up to 2 aluminium insert blocks or one 96-well plate block
• Temperature range from ambient to 100°C or 200°C
• Fast heat-up rate 30°C to 100°C from just 12 minutes
• Temperature stability at 40°C of less than ± 0.1°C

Technical Specification
 

Ordering Information
 

Block inserts need to be purchased separately

Techne TC-312