Homogenisers - High Pressure News

Introduction
IKA HPH 2000/04-DH5 Homogenisers-High Pressure from Wolflabs.
Efficiently produce emulsions with particle sizes in the nano range and perform cell extractions: Such results are made possible by IKA’s high-pressure homogenizers which are capable of operating in continuous in-line operation. For both laboratory and industrial needs, IKA's high pressure homogenizers produce ultra-fine emulsions with extremely narrow droplet distribution and can be used for cell disruption by means of extreme energy input.

Features and benefits
- Highest possible energy input which allows for a particle reduction of up to 0.1 µm
- Proven construction and long service life
- Very low noise level
- Sample volumes from 10 ml processible
- Deliverable in several sizes and versions for flow capacities from 2 to 100 l/h
- Parts that come in contact with the product are made of stainless steel and ceramic

New LiposoFast and EmulsiFlex series of high pressure homogenisers from Avestin have been added. 

Prices starting at £871 - https://www.wolflabs.co.uk/laboratory-products/homogenisers-high-pressure

How to get the best out of your high pressure homogeniser
- It is important that the user identifies the homogenising pressure that will achieve lysis of those particular cells, as pressures that are too low may prove ineffective.
- Pressure and temperature are directly affected by each other. As a general rule, a 1000psi (69bar) increase in pressure results in a 1°C increase in temperature, which could potentially impact homogenisation results negatively – it is therefore important that pressure is controlled.
- Tougher samples generally require higher pressures for processing. In the case of DNA or protein isolation, it is crucial that the optimal homogenising pressure for a particular sample does not induce a temperature high enough to damage sensitive intracellular products.
- Temperature increase may therefore induce an increase in oil viscosity and thus emulsion viscosity, so it can directly and significantly alter the quality of the end product, given optimal viscosity of an emulsion is always crucial. Avestin machines can offer that precise temperature control via compatibility with cooling coils, as well as sanitary heat exchangers.
- It should be noted that some products require high homogenising pressures for processing; however these pressures can damage sensitive products. For example, proteins may be denatured. A solution to this problem lies in lowering the pressure and increasing the number of passes through the machine. Multi-pass, high-pressure homogenisation has been shown to induce higher microbial inactivation than single-pass high-pressure homogenisation of which may be a preference to some.
- In summary, the identification of the optimal parameters when processing samples via high pressure homogenisation, can allow both ease of use and cost-effectiveness.

Introduction
Avestin B15 high pressure homogeniser from Wolflabs. The Emulsiflex B15 from Avestin is a benchtop high pressure homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The B15 is air/gas powered and requires no electrical power to run. The pump is designed to ensure silent operation.

Features and benefits
- Capacity of 2-15ml batch.
- Minimum sample size 2 ml.
- Requires compressed air/gas.
- No electric power needed.
- Suitable for use in cold room.
 

Introduction
Avestin C5 high pressure homogeniser from Wolflabs. The EmulsiFlex-C5 from Avestin is a benchtop homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The unique EmulsiFlex design ensures that there are no "O"-rings or gaskets in the entire path of the product. This means less risk of contamination, easier cleaning and no leakage. Pressures of up to 30,000psi enable the EmulsiFlex-C5 to process the toughest samples. With a footprint of 43x17x31 cm, and weighing only 25kg, the C5 is compact enough to fit onto the most overcrowded lab bench without compromising power or performance.

Features and benefits
- Specially designed pump motor pilot valve ensures quiet operation..
- Pneumatically controlled, dynamic homogenizing valve.
- Easy disassembly for cleaning or inspection..
- Same valve is used in larger models for scalable performance.
- Stringent inlet and outlet temperatures can be controlled with the installation of a cooling coil or heat exchanger.
- Suitable for cold room use.

Introduction
Avestin EmulsiFlex-C3 high pressure homogeniser from Wolflabs. The EmulsiFlex-C3 from Avestin is a benchtop homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The EmulsiFlex-C3 is electrically powered with a pump designed to ensure quiet operation. The unique Emulsiflex design ensures there are no "O"-rings or gaskets in the entire path of the product. This means less risk of contamination, easier cleaning and no leakage.

Features and benefits
- Specially designed pump motor pilot valve ensures quiet operation.
- Pneumatically controlled, dynamic homogenizing valve.
- Easy disassembly for cleaning or inspection.
- Same valve is used in larger models for scalable performance.
- Constant, low-through capacity of up to 3l/hr, depending on homogenising pressure.
- Adjustable homogenizing pressure in the range of 500 - 30,000psi.