Quality Control of your Results
Bio-Tek recognizes the importance of verifying the performance of analytical instrumentation. Many regulatory agencies, laws (such as CLIA 88) and Good Laboratory Practices (GLPs) require a microplate reader be periodically checked to ensure it continues to meet factory specifications.

Bio-Tek Provides Performance Verification
All Bio-Tek automated readers can be quickly and simply tested for critical performance specifications using two complementary methods uniquely developed by Bio-Tek Instruments:

• Bio-Tek's optional Universal Calibration Test Plate uses neutral density filters and precision machined alignment holes to confirm performance specs.
• Bio-Tek's on-board Reader Diagnostic Self-Test verifies the reader's optical and electronic performance.

Autoreader Calibration Test Plate
Bio-Tek's Universal Calibration Test Plate helps meet regulatory instrumentation requirements by testing four critical specifications:

• Alignment
• Accuracy
• Reproducibility
• Linearity

No Liquids or Pipetting
The Universal Calibration Test Plate is the size and shape of a standard microplate and is inserted into the microplate carrier of your autoreader. Within seconds, the four critical parameters described above are analyzed in detail.

NIST-Traceable
Each Test Plate consists of the following:

• NIST (NBS)-traceable neutral density glass filters for checking accuracy, reproducibility, and linearity
• Blanking verification holes
• Alignment verification holes

8-Standard Wavelengths
Exact spectrophotometric calibration data is supplied with every plate for each neutral density filter at 8 wavelengths: 405, 450, 490, 550, 620, 630, 690, and 750 nm.

Use as a Documentation Tool
By providing an easy and convenient method of monitoring the performance of your autoreader over time, the calibration test plate (coupled with the diagnostic reader self-test) can be an invaluable aid in diagnosing system performance, as well as a documentation tool in your research or clinical laboratory.

• Performance criteria and instructions

• Protective case

Optional Accessories

• Factory recertification (recommended once per year)

Bio-Stack^TM is the result of Bio-Tek's years of experience in Microplate automation. With its compact and flexible design, the Bio-Stack is compatible with Bio-Tek's Microplate washers, dispensers and detection systems. Speed, ease of use and walkaway automation are guaranteed for any routine Microplate process. When used with the ELx40 washer or µFill continuous reagent dispenser, the Bio-Stack's small footprint fits easily in most standard laboratory hoods without the need for a computer. As a batch processor for the PowerWave HT, Synergy HT or PowerWave XS Microplate readers, the stacker interfaces seamlessly with Bio-Tek's KC4 Data Analysis Software. The Bio-Stack provides life science laboratories with simple and cost-effective automation for increased throughput in genomics, proteomics and drug discovery applications.

• Automates routine laboratory procedures
• Adds stacking capability to Bio-Tek's robotic Microplate instruments:
  • ELx405^TM Automated Microplate Washer
  • µFill^TM Microplate Reagent Dispenser
  • PowerWave^TM HT Microplate Spectrophotometer
  • PowerWave^TM XS Microplate Spectrophotometer
  • Synergy^TM HT Multi-Detection Microplate Reader
• 30 Microplates per interchangeable stack
• Simple to use for all laboratory personnel
• Restacking capability
• Variable Microplate input
• Small footprint conserves precious Benchtop space
• Universal design compatible with all supported Bio-tek instrumentation
• Aluminum precision-ground base design withstands heavy use
• Linear mechanisms built around proven Bio-Tek technology
• Cams actuated by stepper motors for assured quiet operation
• No moving parts to wear within motors
• Gripper incorporates rack and pinion drive for synchronistic movement
• Gripper spring pre-loaded to close in the event of a power failure and remains fixed in space for no spillage
• Microplate addressed from below for reliable transport
• Sensors verify Microplate presence
• Convenient alignment mechanism allows easy removal and placement of Bio-Stack with no recalibration
• Onboard self diagnostics ensures trouble-free operation

Bio-Stack^TM
Automated Microplate Stacker

• Serial cable: null modem, 25-pin female to 25-pin female
• 2 Microplate stacks
• Operator's manual
• One instrument accessory kit

• ELx405^TM accessory kit
• µFill^TM accessory kit
• PowerWave^TM HT and PowerWave^TM XS accessory kit
• Synergy HT accessory kit

µFill offers an economical, compact, and reliable alternative to existing microplate dispensers. Accurate and precise dispensing are guaranteed without the time-consuming recalibration, cassette replacement, and maintenance commonly associated with other dispensers available today. An autoclavable model is also available for those applications requiring a sterile fluid bath.

Using Bio-Tek's vast expertise in liquid handling, µFill^TM incorporates a microprocessor controlled syringe pump for optimal performance. Both 96- and 384-well standard microplates and deep well blocks are accommodated with flexible volume ranges from 5 µl to 3,000 µl per well. µFill's compact footprint makes it ideally suited to fit inside laminar flow cabinets. The user-controlled dispense flow rates allow low- to high-velocity dispensing for any application. Twenty microliters are dispensed across a 384-well plate in 12 seconds.

• Robot compatible carrier accommodates both 96- and 384- standard and deep well microplates
• Microprocessor-controlled syringe pump guarantees optimal precision and accuracy
• No calibration required
• Low-maintenance design with Bio-Tek's long history of liquid handling instruments
• User-controlled dispense flow rates for low- to high-velocity dispensing
• Up to 75 programs stored in multiple languages at the touch of a button
• Built-in maintenance routines

AF1000
µFill Microplate Reagent Dispenser

AF1000A
µFill Microplate Reagent Dispenser with autoclavable fluid delivery path
Includes autoclavable fluid path for guaranteed sterility.

Language versions available: English, French, German, Italian, and Spanish

• Operator's manual
• Priming trough
• Supply tubing set
• Aerosol cover
• 16-channel dispense manifold
• Maintenance kit

• µFill ActiveX^TM Programmer's Package (7140202)
  Forwards control commands from a PC-based container application to Bio-Tek's µFill. For experienced computer programmers.
• RS-232 serial cable (75053)
• Priming trough draining set (7140006)
• 8-channel autoclavable manifold for 96-well 1/2 area and PCR plates (7140515)
• PCR plate adaptor (6002076)
• Supply tubing set for dispensing organic solvents (7140514)
• Spare autoclavable syringe head with piston (7140516)
• Spare standard 16-channel manifold (7140506)
• Spare autoclavable 16-channel manifold (7140508)
• Spare standard tubing set (7140505)
• Spare autoclavable tubing set (7140510

• Dust cover, p/n 7332040
• Printer cable, p/n 71072
• Operator's manual, p/n 7331000

• Endpoint, kinetic, and multi-wavelength read modes
• Collect and analyze data from multiple microplate geometries
• 7 curve fit types: linear, cubic, cubic spline, 2-p, 4-p, quadratic, and point-to-point
• Extrapolation, standard averaging, and interpolation options
• Kinetic max slope calculation
• Multiple endpoint plates collection into a single result record
• Data transformation and plate formula capability
• Experimental plate data entry
• Customizable well identifiers
• Intuitive user interface for easy system setup and protocol definition
• Reader system test and test plate diagnostics
• Complete archiving utility for program maintenance
• Flexible reporting options
• Extensive context-sensitive help system
• Data import from KCjunior or KCII DOS software

This model includes our patented Dual-Action^TM manifold, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection in both 96- and 384-well microplates. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its slanted dispense tubes, full control of dispense and aspiration rates and unique x, y, z positioning, the ELx405 Select is ideal for cell-based applications.

For those applications that use only 96-well microplates, the ELx405 Auto Plate Washer is an ideal choice for performance and value! The ELx405 Auto Washer is the most precise and reliable washer available. Many unique features lower background absorbance values and increase the signal-to-noise ratio of typiceal ELISA assays. The ELx405 Magna is specifically designed for those applications using magnetic bead technology. A second carrier positions the magnet plate close to the microplate bottom, securing magnetic beads during the critical aspiration cycles. All ELx405 Series washers come standard with Bio-Tek's unique AutoPrime^TM and AutoRinse^TM functions for reliable performance, and can be interfaced into standard robotic systems with Bio-Tek's easy-to-use ActiveX® component.

ELx50/16
384-well and flat-bottom 96-well paltes and strips (1x8)

ELx50/16V
384-well and flat-bottom 96-well plates and strips (1x8) with valves

ELx50/8
96-well plates and strips (1x8)

ELx50/8V
96-well plates and strips (1x8) with valves

ELx50/12
96-well plates and strips (1x12)

ELx50/12V
96-well plates and strips (1x12) with valves

• 8-, 12-, or 16-channel manifold
• Tubing set
• Wash, rinse, and waste bottles
• Maintenance kit
• Vacuum filter
• Internal fluid delivery and vacuum system
• 24-volt power supply
• Operator's manual
• Short form instructions
• Bio-Tek provides with each washer its own QC report and performance verification data sheet. This sheet reports the evacuation efficiency, dispense accuracy, and precision for the specific washer shipped.

• 8-channel manifold, p/n 4070512S
• 12-channel manifold, p/n 4070513S
• 2-liter supply bottle with cap and connector, p/n 4070515
• 2-liter waste bottle without cap, p/n 48140
• Supply tubing set, p/n 4070510
• Waste tubing set includes rubber stopper, p/n 4070511
• Vacuum line filter, p/n 48146
• Stylus maintenance kit, p/n 4070523
• 8-channel, short-dispense tube manifold, p/n 4070519
• Aerosol cover, p/n 4070517

* 8-channel manifold accessory available for round- and V-bottom plates.

Bio-Tek is now offering Instrument Validation Packages. Each validation package includes thorough procedures to allow a user to perform Installation Qualification, Operational Qualification and Performance Qualification. Periodic maintenance procedures are also included to assist in maintaining the instruments for optimal performance.

The following packages are available, or soon to be released.

ELx808 validation package (pn 7340543)

FLx800 validation package (pn 7080532)

PowerWave X validation package (pn 7260544)

PowerWave HT validation package (pn 7280520)

ELx405 validation package (pn 7101033)

*The Universal Test Plate (pn 7260522) is required in order to run some of the procedures specified for the absorbance readers.

LabRack

Laboratory Instrument Rack

Overview

• Speed: 5 wash/aspirate cycles in 5 seconds, one row at a time
• Fluid Dispensing: Gravity-fed, user-controlled fill volumes with convenient finger-controlled valve
• Suction Efficiency: 6 microliters maximum residue/well
• Safety: Overflow protection for flat and U-bottom polystyrene microplates
• Operation:
  • Dispense: User-controllable dispense valve
  • Aspirate: Continuous
• Maintenance: All chemically-resistant coatings and materials; readily cleaned and completely autoclavable

EL40108
8-channel washer
EL40112
12-channel washer

• Operator's manual

• Alignment assembly, p/n EL40100
• 8-channel microplate washer manifold, p/n EL40108
• 12-channel microplate washer manifold, p/n EL40112
• Accessory kit with pump, p/n 4010052 (110v); p/n 4010053 (220v)
• Accessory kit without pump, p/n 4010028

Bio-Tek’s Fastrack is an automated and flexible robotic microplate stacking system with pick & place capability. Designed to complement our existing product line of microplate readers, washers and automated pipetting systems, Fastrack can be interfaced to one or more Bio-Tek products. The result is an integrated microplate stacking system for unattended operation. Total Control Software makes this integration simple and intuitive! Fastrack’s unique microplate rotational gripper and 345^o axis of rotation add flexibility and eliminate time consuming alignment.

The Fastrack product line has been expanded to include models equipped with a telescoping arm. In addition to the robot arm's 345^o axis of rotation and rotational gripper, the telescoping arm further increases the robot's flexibility. With this new feature, customers can place Bio-Tek microplate instruments nearly anywhere within the robot's reach!

 

• Interfaces with Bio-Tek readers, washers and liquid handling systems
• Adds capacity to single modules
• 30 Microtiter plates per stack, 360 plate maximum capacity
• Links multiple instruments to create a complete system
• 345^o Axis of Rotation maximizes capacity
• Rotational gripper allows for ultimate configuration flexibility
• Telescoping arm for increased instrument access
• Portable/Small footprint
• Add automated plate-sstacking capacity to single readers/washers and pipetting systems for unattended operation
• Complete assays can be performed by linking individual components with one Fastrack
• High throughput can be achieved by linking the same multiple modules to Fastrack in series (Example: multiple microplate washers with one Fastrack)
• Cost-effective alternative to linear track and stationary robot systems

Fastrack
Standard arm

Fastrack TD
Standard arm with deep well plate capability

Fastrack XL
Extended (4") arm for increased instrument accessibility

Fastrack XLTD
Extended arm with deep well plate capability

NEW! Fastrack XT
Telescoping arm

NEW! Fastrack XTTD
Telescoping arm with deep well plate capability

NEW! Fastrack XTRA
Telescoping arm with random access feature

• Serial cable: 9-pin male to 9-pin female
• Stack alignment base
• 2 microplate stacks (3 with TD versions)
• Microsoft® Windows® operating software
• Operator's manual

• Barcode reader
• Deep well plate and tip transport
• Instrument alignment bases
• Instrument interface software

The FLx800 is designed to combine excellent specifications and performance with convenience and ease of use. This design incorporates powerful performance at a price much lower than traditional fluorescence microplate readers. The FLx800 line includes several models with options that meet the specific needs of research and OEM users, including the ability to read multiple plate types and PCR tubes. New models in the FLx800 family include a reagent dispenser module. This new external dispenser module contains a precision syringe designed to deliver reagents directly into the wells of a 6-, 12-, 24-, 48- or 96-well microplate. The injector position can be optimized for detection from the top or bottom of the microplate for increased application flexibility. The injector model increases the range of applications available, including important calcium updatake reactions, and expands the range of high sensitivity microplate fluorescence instrumentation offered by Bio-Tek.

The FLx800 has on-board data reduction software or can be fully computer controllable via Bio-Tek’s powerful and easy-to-use KCjunior^TM or KC4^TM PC software.

• Custom-mapped fiber optics for optimal signal transfer
• Selectable, automated top- or bottom-probe reading for best sensitivity
• Typical fluorescence sensitivity to 5 pg sodium fluorescein
• Glow luminescence reading with top detection for best sensitivity
• Incubation to 50^oC for temperature-sensitive assays
• Wavelength range from 350-800 nm allows detection of many common fluorophores
• PCR tube-reading option available
• Reads 6- to 384-well formats, Terasaki trays and PCR plates
• Convenient front panel accessory access makes changing filters simple
• Powerful on-board data reduction software
• Computer control via KC4 or KCjunior software

All models come with 2 excitation and 2 emission filters.

FLx800T
Top probe (3mm) only

FLx800B
Bottom probe (5mm) only

FLx800TB
Top and bottom probes (3mm and 5mm)

FLx800TBI
Top and bottom probes (3mm and 5mm), incubation to 50^oC

FLx800TBIE
Top and bottom probes (3mm and 5mm), extended range PMT, incubation to 50^oC and shaking

FLx800TBE
Top and bottom probes (3mm and 5mm), extended range PMT

FLx800TBID
Same as FLx800TBI, includes single reagent dispenser

FLx800TBIDE
Same as FLx800TBIE, includes single reagent dispenser

FLx800TBEP
Top and bottom probes (3mm and 5mm), extended range PMT, top/bottom PCR tube and microplate reading capability

• 2 Excitation filters (485/20, 360/40)
• 2 Emission filters (528/20, 460/40)
• Serial cable: null modem, 9-pin female to 25-pin female
• Operator's manual

• KCjunior^TM software
• KC4^TM software
• See price list for available filters

Bio-Tek's EL301 Microwell Strip Reader is a portable, rugged, single/dual wavelength photometer designed to measure and interpret the absorbance's of microwell strip assays.

Flexible enough to accommodate flat or round bottom 8-well, 12-well, and 2 x 8-well strips, the EL301 provides an affordable alternative for all your qualitative assays, as well as the precision, accuracy, and data processing capability required for quantitative clinical tests.

The on-board microprocessing system allows you to choose from four internal user-defined computer programs, each accessed with the touch of a pad:

• Single or Dual Wavelength: Display absorbances
• Linear Regression: Enter up to 5 standards to determine sample concentrations.
• Limit Thresholds: Define cutoff values to determine ± results of absorbances or concentrations.
• Difference: Determine delta absorbances.

Well locations, absorbance values, and results are displayed on the large LCD display. Standards and blanks may be carried over from strip to strip within each program. Data may be stored in the EL301's memory, printed out, and sent to a computer for more detailed analysis. Up to 12 plates (1152 wells) can be stored in memory.

• Automatic well location
• 4 internal user-defined analysis programs
• Automatic average blanking
• Large 2-line, 16-character LCD for user prompting and data entry
• Internal memory stores up to 12 plates of absorbance values
• RS-232 and parallel printer interfaces
• Battery or line powered

• Molded carrier assembly, p/n 3010513
• 3 strip holders: 1 x 8, p/n 3010515; 1 x 12, p/n 3010516; 2 x 8, p/n 3010517
• Lamp assembly, p/n 3010502
• Bulb wrenches (2) (call for part number)
• Power transformer and cord (call for part number)
• Printer cable, p/n 3011509

• Battery pack, p/n 3011513
• Carrying case, p/n 3012085
• 1 x 12 breakaway strip holder, p/n 3102077
• Citizen printer or equivalent (110v only), p/n 97128
• Citizen printer paper roll, p/n 97129
• EL301 filters, p/n 3014---- (plus wavelength)
  • 405, 410, 415, 450, 490, 510, 515, 540, 550, 560, 570

   

• 340-999 nm - without filters
• Temperature control to 50^oC
• KCjunior data reduction
• Spectral scanning
• Pathlength correction via KCjunior

PowerWave_X 340 (PRWUIE)
340-999nm wavelength range, onboard spectral scanning 4-Zone^TM temperature control to 50^oC, and KCjunior software.

• Operator's manual
• Assay Reference Guide
• Printer cable
• Serial cable: null modem, 9-pin female to 25-pin female
• KCjunior software

• Patented Bio-Cell^TM
• Bio-Cell adapter plate assembly
• Universal Test Plate
• Parallel printer cable: 25-pin male to 36-pin male
• KC4^TM software

• Monochromator optical design with powerful xenon flash lamp
• Select wavelengths from 200 to 999 nm in 1 nm increments without the use of filters
• 96- and 384-well microplate or 1 cm Bio-Cell^TM reading for application flexibility
• 4-Zone^TM temperature control to 50^oC provides superior temperature stability
• Ultra fast read times for 96- and 384-well formats
• Small footprint and freely accessible carrier for easy integration into automated systems
• With pathlength correction, easily convert cuvette-based assays onto a microplate format
• Versatile KC4 v3.0 software with exclusive PowerReports^TM feature for unique report customization.

PowerWave HT (RPRW)
200-999 nm, 96- and 384-well plate reading with programmable shaking. Includes KC4 v3.0.

PowerWave HT with Incubation (RPRWI)
Standard PowerWave HT, plus temperature control from ambient +4°C to 50°C. Includes KC4 v3.0.

• KC4 Software
  KC4 is the primary operating system for the PowerWave HT for extensive data reduction and custom reporting via Power Reports™.
• Serial cable: null modem, 9-pin female to 25-pin female
• Operator's manual

 

• Universal Test Plate; p/n 7260522
  For verifying the accuracy, linearity and repeatability performance of the PowerWave HT.
• ActiveX® component
  Contains all information required for external control of the reader functions. Help System included.
• KCjunior software; p/n 5270501
  Alternative to KC4 for control of the PowerWave HT
• Patented BioCell^TM 1 cm vessel; p/n 7272051
• Bio-Cell adapter plate; p/n 7270512

• 96-well microplate and 1 cm reading (Bio-Cell^TM)
• Continuous wavelength selection from 200-999 nm without filters
• Monochromator design with high-powered xenon flash lamp
• 4-Zone^TM temperature control to 50^oC and programmable shaking for critical kinetic assays
• Automated pathlength correction for direct nucleic acid quantitation
• Pre-programmed assays on-board, plus open protocols for user-defined assays
• Plate reads in as few as eight seconds
• Easily convert cuvette assays to a microplate format

• Operator's manual
• Assay Reference Guide
• Printer cable
• Serial cable: null modem, 9-pin female to 25-pin female
• KCjunior^TM software

• Patented Bio-Cell, p/n 7272051
• Bio-Cell adapter plate assembly (see photo below), p/n 7270512
• Universal Test Plate, p/n 7260522
• Parallel printer cable: 25-pin male to 36-pin male, p/n 71072
• Serial cable, null modem: 9-pin female to 25-pin female, p/n 75053
• Serial cable adapter: 9-pin male to 25-pin female, p/n 49755
• Macintosh cable: 8-pin round to 25-pin female, p/n 75019
• Epson LX300 printer or equivalent (110v only), p/n 97145
• Hewlett Packard Deskjet printer(110v only)
• KC4 software
• KCjunior software

• Reagent addition
• Mixing within a well
• Serial dilutions
• Rapid Dispense Mode
• Plate replication
• Sample transfer
  • Plate-to-plate (96 to 96, 384 to 384, 96 to 384)
  • Within a plate

• Pipette:
  Precision 2000's uniquely designed pipette is made up of 12 or 8 individual syringes and guarantees reliable tip pickup. Each syringe is free floating within the body of the pipette. When tips are picked, the syringes are free to move up to accommodate differences in height and radially to accommodate deviations from ideal position. Precision 2000 achieves the most reliable seal on all tips with equal force.
• Work Surface:
  Precision 2000 comes equipped with a user configurable platform. A reagent trough, disposable tip container and up to six 96- or 384-well microplates can all be positioned where they best suit your application. The entire platform can be removed for easy cleaning. When pipette tips are used, a programmer can decide whether to return the tips to the source for reuse or discard in the waste vessel provided.
• Robotic Compatibility:
  Robotic compatibility is a standard feature with Precision 2000. The configuration of the platform combined with its XY transport capability make Precision 2000 compatible with robotic plate handlers. An ActiveX^TM package is available for simple integration.
• Rapid Dispense Mode:
  Precision 2000 ensures fast, flexible and precise automated liquid delivery. Rapid Dispense Mode is made possible with an optional manifold. A separate positive displacement pump allows for the continuous rapid dispensing of reagent from any size reservoir to both 96- and 384-well microplates.
• Safety:
  Precision 2000 optimizes safety in today's laboratory. As an automated pipetting system, Precision 2000 helps eliminate repetitive motion injuries. Its compact size makes Precision 2000 ideally suited for installation into a biological safety cabinet. When installed on the lab bench, an optional protective cabinet is also available to prevent aerosols. This protective cabinet shields the operator while Precision 2000 is running. An internal software safeguard is available at any point in the liquid transfer if a pause or program abort is necessary.

Software:

• Pre-Defined Demo Programs, Program Creation Ability:
  Precision 2000 comes pre-installed with some of the most common pipetting routines, including reagent additions, serial dilutions and plate-to-plate transfers. The software is thoughtfully designed to enable the user to copy an existing demonstration protocol and simply modify it to meet specific requirements. Up to 80 program files can be stored in memory. The user has the ability to create, edit, copy and delete files from the external keypad with a 2 x 24 character display. Virtually any liquid transfer parameter can be customized. Configurable aspiration depth ensures that tips don't touch the bottom of wells while managing sensitive cell assays.
• Validation, Printing, Lock Feature:
  Precision 2000 offers an automatic validation utility which ensures proper creation or modification of protocols before any liquid transfer can take place. This feature conserves time and expensive supplies. After a protocol is created or modified, parameters can easily be printed for an additional verification. A convenient lock feature is also available for security purposes.
• QC Programs:
  Precision 2000 comes equipped with pre-programmed protocols for Installation Qualification, Operational Qualification and Performance Verification needs. Each instrument is factory tested for optimal precision and accuracy performance. Performance documentation accompanies each instrument, and tests can be easily duplicated by the user.

• 4 microplate holders
• 3 pipette tip box/reagent trough holders
• 4 50 ml reagent troughs
• Parallel printer cable: 25-pin male to 36-pin male
• Serial cable: null modem, 9-pin female to 25-pin female
• Maintenance kit
• Operator's manual

• Protective aerosol cabinet
• Precision 2000 pipette tips
• Many additional labware adapters are available. Inquire for more details.
• Precision Power^TM Software
  Precision Power^TM is Bio-Tek’s Windows PC software for complete Precision 2000 control. The graphical user interface simplifies program creation under Windows 95, 98, NT, 2000 and XP. Programs can either be created and run from the PC or downloaded and run from Precision 2000’s onboard microprocessor. Precision Power expands the instrument's dynamic capabilities with features such as a graphical simulation utility, nested looping, and the ability to replenish supplies mid run.
  
  Precision Power PC Requirements:
  • Intel Pentium processor/133 MHz (or compatible)
  • Microsoft Windows 95, 98, 2000, NT (Service Pack #6) or XP operating systems
  • 32MB of RAM minimum
  • 60MB of available hard drive space
  • CD ROM drive
  • SVGA color monitor (800x600 resolution with 256 colors)
  • Keyboard, mouse and serial ports

• Precision Power ActiveX Programmer's package

• Display real-time kinetic rate data for all 96-well plates as it is generated.
• Evaluate each well individually for calculation of rate. Single or multiple data points can be deleted in a single well.
• Multi-tasking capability allows long experiments to be done.
• Select ranges for data analysis before or after data is collected.
• The FIND MAX RATE option allows the automatic determination of the interval with the maximum slope for each well.
• Zoom in on a range of wells or an individual well for ease of viewing and analysis.
• Calculate many optional kinetic parameters including rate, time to max rate, time to onset, and 50% OD.

Easy Protocol Generation

• Protocol files which include specified options for a particular assay can be named and saved separately.
• Store multiple protocols in the computer's memory for easy recall and setup of assays.

Simplified Template Setup

• Define standards, blanks, or samples in any pattern on the plate, including multiple experiments on a single plate.
• Enter multiple dilutions for samples including any desired dilution range.
• Fill in and edit well IDs for the plate automatically, as desired.

Data Analysis and Formula Transformations

• Select from six curve fitting options: linear, semi-log, log/log, log/logit, 4-parameter, and spline for graphing the standard curve.
• Interpolate samples using standard curve stored in memory or from a previous plate.
• Calculated sample concentrations with a complete statistical analysis.
• Analyze samples by comparison to a control using easy formula transformations. Display results in either tabular or plate format.
• Mask and unmask points easily to exclude outliers from data analysis.

Design Your Reports

• Customize the reports to include only the information you want.
• For convenience, the number format can be selected as scientific, decimal, or mixed.
• Print scan data in a tabular format, organized from highest to lowest absorbance values.
• Time to onset, time to peak, correlation coefficients for individual kinetic plots, or any of the kinetic parameters are available in tabular reports.

Endotoxin Applications

• Calculate spike recovery and time to onset easily

Versatile Export/Import Features

• Export kinetic, endpoint, or multiple plate data in a variety of formats, including well-by-well format of 8 x 12 geometrics. Also export any calculated data, if desired.
• Import kinetic, endpoint, or multiple plate endpoint data from previous versions of Delta-Soft directly or from any spreadsheet or other program as text files.
• Interfaces with all Bio-Tek EL300 and EL800/808 series readers.

• Operator's manual
• Serial cable, Part #3070145

KC4 Signature

21 CFR Part 11 Compliant!

Overview

Specifications

Recommended System Configuration Pentium II processor, 300 MHz minimum Microsoft® Windows® 98/NT/2000/XP operating system 128 MB RAM 30 MB of available hard drive space for program files CD ROM drive Keyboard and mouse Serial port (or USB port with serial adapter) To use KC4's PowerReports feature: Microsoft Office 2000

Bio-Tek’s KC4 v3.1 is the most innovative tool available to create custom, publication quality reports of microplate applications. KC4’s PowerReports^TM feature uses true Object Linking and Embedding (OLE) technology to interact with Microsoft’s Excel and Word programs, providing total control over all report formatting using data objects created by KC4. In addition to the exciting PowerReports^TM feature, KC4 includes these capabilities:

• real-time kinetic well zoom for easy assay monitoring
• fully customizable plate identifiers for intuitive template creation
• enhanced area scanning graphics and reporting
• custom configurable symbols for cutoff reports
• direct export to Excel or Word
• data audit trails

KC4 v3.1 Software, Part #5290512

• Software on CD
• Operator's manual
• Single user license

KC4 has powerful, flexible data manipulation capabilities and is suitable for all applications, such as:

• Research
• Nucleic acid quantitation
• Drug Discovery
• Fluorescence, luminescence assays
• Proteomics and Genomics
• Enzyme kinetics

Today's research requires that instrumentation be cost effective, reliable and easy to use. In response to those requirements, Bio-Tek Instruments has developed the Synergy^TM HT. This new microplate reader for fluorescence and absorbance measurements was designed with an emphasis on superior performance in both detection methods as well as sensitive glow luminescence measurements. Unlike many multi-detection microplate readers, the Synergy HT utilizes a unique dual-optics design. When making fluorescence determinations, the Synergy HT uses a tungsten halogen lamp with interference filters for wavelength specificity in conjunction with a PMT detector. The tungsten halogen lamp produces a large amount of light at a constant intensity, providing increased sensitivity and repeatability. Specially designed fluorescent interference filters prevent light leakage from excitation wavelength to the emission wavelength, particularly important with fluorescent compounds with small Stoke's shifts. In addition, the ability to use filters with different bandpasses allows the investigator to tailor their fluorescence measurements to their experiment more effectively. When absorbance measurements are made, the instrument switches to a xenon flash lamp and a monochromator for wavelength selection. The use of a xenon flash lamp allows for both UV and visible light absorbance measurements. The monochromator provides wavelength selection from 200 to 999 nm in 1 nm increments. Thus, dual-optics design allows the investigator to have two different reading modes without compromise.

Synergy HT reader has a 4-Zone^TM temperature control option which ensures superior temperature uniformity necessary for kinetic assays. With a compact footprint and robot-accessible carrier design, the Synergy HT is also compatible with most automated systems. Reader control is via Bio-Tek's KC4 data reduction software or an ActiveX® software component.

• Automatic top and bottom epifluorescence read modes optimized for both sensitive homogeneous and cell-based assays
• Software-controlled automatic probe height adjustment with built-in plate sensor to prevent probe crashes
• Scanning monochromator for wavelength scans to identify peak absorbance and provide full spectral analysis
• Luminescence measurements with top detection for best sensitivity
• Reads 6- to 384-well plate formats including PCR trays and Terasaki plates
• Well area scanning for popular cell-based assays
• Time Resolved Fluorescence capability
• Includes powerful KC4^TM PC software with exclusive PowerReports^TM feature for custom reporting

• KC4^TM v3.0 with PowerReports^TM
• Accessory kit
• Serial cable: null modem, 9-pin female to 25-pin female
• Operator's manual

The PowerWave^TM XS is the newest edition to the powerful PowerWave line of products and sets a new standard for performance and value. Never has this line-up of features been so affordably priced. Any microplate type from 6- to 384-wells is accommodated including Terasaki formats and Bio-Tek’s patented Bio-Cell^TM quartz vessel for standard 1 cm measurements. A variety of read modes including endpoint, kinetic and spectral scanning assays are available. Applications include ELISA, kinetic enzyme assays, nucleic acid quantitation, A₂₆₀/A₂₈₀ ratios, protein analysis and much more.

The PowerWave XS offers tunable wavelength selection from 200 to 999 nm in 1 nm increments and wavelength scanning without the need for filters. Unique 4-Zone^TM temperature control to 50°C, user programmable shaking, robotic compatibility and ultra fast Sweep read mode are standard features of the PowerWave XS. The instrument’s small footprint is designed to conserve precious bench top space.

• No Interference Filters Required
  The PowerWave XS is equipped with a monochromator for continuous wavelength selection from 200 – 999 nm. Wavelengths can be selected in 1 nm increments to meet all assay requirements. It is like having 800 filters built in. If the best conditions are not known, any well can be spectrally scanned for a portion of the spectrum or across the entire wavelength range.Mutiple Microplate Types
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• Any standard microplate from 6- to 384-wells can be accommodated. This includes 60-, 72- and 96-well Terasaki plates. The PowerWave XS is truly a universal microplate spectrophotometer. In addition to microplates, the PowerWave XS can also read up to 8 of Bio-Tek’s patented Bio-Cell^TM quartz vessels for fixed 1 cm measurements.
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• Choice of Reading Speeds
  The PowerWave XS offers speed options based on the customer’s requirements. Normal Mode incorporates Bio-Tek’s enhanced reading capabilities. With the use of a xenon flash lamp, every well is flashed 8 times. If the average of the 8 wells is less than 2.0 OD, the result is reported. If the average is greater than 2.0 OD, the well is flashed a total of 64 times. An average of the 64 reads is accurately reported for an assured result. In Rapid Mode, every well is flashed 8 times regardless of the result. If higher throughput is required, the PowerWave XS’ Sweep Mode meets the challenge. Using “on the fly” technology, an entire 96-well plate can be read in 14 seconds. Even when spectrally scanning a well, the user has a choice of reading speeds.
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• Temperature Control and Microplate Shaking
  Bio-Tek’s 4-Zone^TM temperature control is a standard feature with the PowerWave XS. Unlike a forced hot air system, the PowerWave XS incorporates a natural convection system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50° Celsius. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters guaranteeing no more variation across the microplate than ± .5° at 37° Celsius. The PowerWave XS allows users to select from four shaking intensities for controlled agitation. Low, medium, high and variable intensities can be chosen in addition to the shaking duration.
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• KCjunior Software Included
  Bio-Tek’s KCjunior software is packaged with the PowerWave XS at no addition charge. KCjunior provides complete control in an intuitive and user-friendly format.

• KCjunior^TM Basic Data Analysis and Curve Fitting Software
• Operator's manual
• Serial cable
• Power cord

• Universal Test Plate
• Bio-Cell^TM Quartz Vessel
• Bio-Cell Plate Adapter
• Adapter for 60-/72-/96-well Terasaki microplates
• KC4 v3.0 Software

• Extensive on-board data analysis, including
  • Data transformation formulas
  • Assay and control validation
  • Curve-fitting options: linear, 4-P, 2-P (logit/log), cubic, quadratic, cubic spline, point-to-point
  • Multiple assays per plate
• Fast Kinetics
  • Measurements in intervals as short as 6 seconds
  • Kinetic rate, R² or onset time reported without needing a PC
• Linear well scanning for agglutination assays
• 4-Zone^TM Temperature Control
  • Incubation to 50^oC
  • Improved reproducibility in enzyme kinetic reactions
  • Enhanced uniformity through independently monitored heaters
  • Unique heated track/carrier design for minimal evaporation and edge effect
• Superior optical design
  • Incorporates a staggered optical design to eliminate channel-to-channel crosstalk
  • Reference channel rolls optical signal for uniform channel-to-channel signal

• 405, 450, 490, 630 nm filters
• Dust cover
• Parallel printer cable: 25-pin male to 36-pin male
• Operator's manual
• Serial cable, null modem: 9-pin female to 25-pin female

• Patented Bio-Cell quartz mini-cuvette: to obtain 1 cm fixed path length absorbance measurements
• Bio-Cell adapter plate assembly: can contain up to 8 Bio-Cells
• Universal Test Plate
• Serial cable adapter: 9-pin male to 25-pin female
• Macintosh cable: 8-pin round to 25-pin female
• Epson LX300 printer or equivalent (110v only)
• Hewlett Packard DeskJet Printer(110v only)
• Lamp for ELx808 (all models)
• ELx808 filters (see price list)
• Cytochrome C filter (550 nm, 1 nm bandwidth)

• KC4 software
• KCjunior software
• Delta-Soft Macintosh software

• Agricultural
• Biotechnology research
• Cell biology
• Cell counting
• Cell proliferation
• Clinical
• ELISA
• Environmental
• Food processing
• Kinetic ELISA
• LAL
• Neuroscience