Freeze Dryers - Christ

(DELTA 2-24)

Highest level of process-control and reproducibility for rapid, safe freeze drying of your high-quality products.

With 50 years of experience, Martin Christ GmbH is the leading producer of, not only routine-process freeze dryers, but also of a series of product-specific, highly specialized freeze dryers with far reaching potential. This line of equipment is distinguished by its innovative Lyo-Screen-Control (LSC) interface, its exceptional design and the most advanced level of refrigeration and vacuum technology. The freeze dryers are available as laboratory freeze dryers with cylindrical drying chambers and pilot systems with rectangular cross-section drying chambers. A practical and diverse collection of accessories permits a wide range of tasks. Outstanding products, superior technology and full customer support. Applying advanced processes to valuable products requires specific system configuration. We offer extensive advice in selecting a system and, on request, will run a freeze-drying test in our applications laboratory. Martin Christ's high-quality freeze dryers are equipped with a modern, intuitive interface known as the Lyo-Screen-Control(LSC). This interface is used throughout our line of laboratory and pilot freeze dryers. It allows for up to 30 different drying programs with even the smallest machine as well as for the connection to a PC for process documentation and process control.

The best in specific systems and process monitoring.

Freeze dryers are increasingly being used for small batches whose production involves detailed information to create validation documents (IQ/OQ documentation) and to prepare monitoring tools that ensure flawless product quality. We not only use standard measurements of product temperature and pressure, but also measurements of electrical resistance (Lyo-control) and weight-change (weighing cell) during the drying process. Optimal product safety can thus be guaranteed. Unacceptable structural changes including the collapse of the matrix, i.e. possible cross-contamination during the drying phase, can be avoided with confidence..

Laboratory Freeze Dryers

The optimal system for advanced applications:
• compact, high-performance laboratory and technical equipmentrequiring minimal space
• modular systems permit capacity expansion with a wide varietyof accessories
• complete processing of entire freeze-drying cycle in ice condenser chamber, program controlled, if desired, from-30°C (prefreezing) with single-stage compressors, to -50°C(prefreezing) with doublestage compressors
• innovative, easy-to-use interface LSC with PC connection for process control and documentation
• freeze-dryer system fulfills FDA and cGMP-Guidelines, interfacefulfills the current requirements according to GAMP, Lyo-software LPC-16 also meets US CFR requirements
• drying chamber is located directly above the ice condenser chamber; maximum cross-sectional area results in high sublimation performance and shortest possible process-time
• ice condenser chamber with inner condenser coil of high- quality steel allows for efficient vapour deposition
• thermal insulation of ice condenser chamber avoids power loss and eliminates build-up of water condensation
• ice condenser can be checked visually
• easily accessible ice condenser; rapid, easy defrosting and cleaning (electrical or hot-gas defrosting available); mechanicalor electric defrosting drain
• chemically resistant systems for freeze drying solvents (e.g. DMSO, Acetonitrile, Tert-Butanol, Dioxane) are available
• Windows-based software LPC-16 (Lyophilization Process Control)for PC process control and documentation available with all systems
• simple alternative process documentation using Martin Christprinter or Lyolog LL-1 software
• when required, system qualification (IQ/OQ documentation) for validation purposes

Pilot Freeze Dryers

The highest level of research and development for newproducts.

The Epsilon 1-4 / 2-4 and Epsilon 1-6 D / 2-6 D set the standard for pilot systems of the highest quality and performance. They share a geometrical likeness with large production machines and use similar temperature-controlling systems. The Epsilon 1-4 / 2-4 units have an ice condenser temperature with a minimum of -55°C and -85°C respectively and is available with one shelf with 0.11 m2 usable surface area. The anodized Aluminium shelves can be directly heated or cooled, making the Epsilon 1-4 / 2-4 suitable for both experimental and routine freeze drying. The two larger models, Epsilon 1-6 D and 2-6 D, are characterized by their drying potential on a maximum of four shelves with an area of 0.28 m2, their distance between shelves can reach 75mm(with three shelves). Due to the temperature control of the stainless steel shelves (via a synthetic heat-transfer medium), these pilot systems fulfil the highest standards of the pharmaceutical and biotech industries. New cooling techniques allow for shelf temperatures of -40°C and -55°C/-70°C (pre-freezing) in the very compact chambers of the Epsilon 1-4 and 1-6 D and the Epsilon 2-4 and 2-6 D, respectively. This permits extremely sensitive pharmaceutical and biotech products, e.g. amorphous structures with a low glass transition point, to be freeze dried safely. These pilot dryers are also available with liquid nitrogen cooling. Simple, process-specific documentation is possible on all systems as a result of the new Lyo-Screen-Control and Lyolog interface. The innovative weighing cell provides the ability to monitor freeze drying in vials and trays, in addition to the standard measurements of product temperature and pressure. An added option is the Windows-based LPC-16 software for PC process control and documentation. LPC-16 is widely used in large freeze-drying production systems and follows the GAMP and cGMP guidelines. Other cGMP guidelines are also followed including maintenance and surface guidelines. The systems can be H2O2 sterilized and can be delivered with an Installation/Operation qualification. All systems are very flexible; all are available with manual stoppering devices and additional manifolds for lyophilization in round bottom flasks. These systems will contribute to the success of your small batch production and product development.

Technical Information

Model	EPSILON 1-4	EPSILON 2-4	EPSILON 1-6D	EPSILON 2-6D
Ice condenser capacity	max. 4 kg	max. 4 kg	max. 6 kg	max. 6 kg
Ice condenser performance	max. 3 kg/24 h	max. 3 kg/24 h	max. 4 kg/24 h	max. 4 kg/24 h
Ice condenser temperature	approx. -55°C	approx. -85°C	approx. -55°C	approx. -85°C
Shelf temperature control (T in °C)	-40 (prefreezing) to +60	-70 (prefreezing) to +60	-40 (prefreezing) to +60	-50 (prefreezing) to +60
Shelves - quantity	1	1	4+1 max.	4+1 max.
Shelf measurements (w x d, mm)	270 x 400	270 x 400	225 x 300	225 x 300
Area (m2)	0.11	0.11	0.07 - 0.28	0.07 - 0.28
Distance between shelves (mm)	135	135	73mm (3+1)	73mm (3+1)
Sealing of vials in vacuum / N2	Optional	Optional	Optional	Optional
Flask drying	Manifold with e.g. 4 controllable vacuum connections each (optional)		Manifold with e.g. 8 controllable vacuum connections each (optional)
Sterilization (H2O2)	Optional	Optional	Optional	Optional
Refrigeration compressor Number x KW	1 x 0.6	2 x 0.6	1 x 0.6 + 1 x 0.5	1 x 0.5 + 2 x 0.6
Type	air-cooled	air-cooled	air-cooled	air-cooled
Dimensions (w x d x h, mm)	780x480x ca.520	780x480x ca.520	860x650x ca.1100	860x650x ca.1100
Electrical connection (V /Hz / kVA)	230/50/3	230/50/3	3x400/50/3	3x400/50/3
Weight (kg)	ca. 175	ca. 205	ca. 310	ca. 330
Interface	LSC	LSC	LSC	LSC
Vacuum display
Vacuum control function
Temperature display:
- Ice condenser (display)
- Shelves (display and control)
- product (measurement of 3 probes max.)
Lyo Control	Optional	Optional	Optional	Optional
Determining end of drying:
- Product temperature measurement
- Pressure-increase measurement			Optional	Optional
Weighing cell	Optional	Optional	Optional	Optional
Manipulator for sample-retrieval during process			Optional	Optional
Programmer module
PC-Interface
PC-Documentations software Lyo LL-1
Process-control system LPC-16	Optional	Optional	Optional	Optional

Freeze Dryers - Christ

(EPSILON 2-10D)

The freeze dryer EPSILON 2-10D LSC is a high performance universal laboratory and pilot unit for freeze drying of solid or liquid products in ampoules, vials, glass flasks, plasma bottles or dishes.

All operations are possible inside the unit:

• Pre-freezing of products on liquid controlled shelves down to a shelf temperature of approx. – 55°C according to preselected desired time and temperature limits.
• Freeze drying (sublimation) of products according to preselected desired time, temperature and pressure limits. Shelf temperature at the initial phase of the sublimation process is approx. – 55°C
• Final drying of products with preselected desired time and temperature limits and high final vacuum to remove the capillary and molecular bounded water. Final vacuum is approx. 0.001 mbar (depending on pump performance).

The freeze dryer EPSILON 2-10D LSC is suitable for freeze drying of materials such as bacteria and virus cultures, blood plasma, serum fractions, antibodies, sera, vaccines, pharmaceutical products such as chloramphenicol, streptomycin, vitamins, ferments, plant extracts for biochemical test.

Double-chamber system:

• Ice condenser directly behind the drying chamber
• Large cross-section between drying- and ice condenser chamber
• Nearly no drop of pressure between both chambers
• Excellent drying rates for sensitive materials and products with low-eutectic points or low collapse temperatures
• High efficiency and economic operation of the freeze dryer
• Application-oriented process-control system LSC (“Lyo-Screen-Control”), microprocessor-based
• Minimized space requirements.

Technical Information

Freeze Dryers - Christ

(ALPHA 1-2)

Experience, expertise, adaptability and flexibility for all freeze-drying procedures.

Based on over 55 years experience, Martin Christ offers a comprehensive range of products for both routine and specialized freeze-drying procedures. The diverse and laboratory-tested accessory line allows the basic unit to easily handle a variety of routine and advanced processes.

Guaranteed consistent results for routine freeze drying Routine processes place continual demands on the operating system. A high degree of consistent, user-friendly, intuitive technical functions and operational flexibility are expected features. The basic equipment of the Alpha and Beta series, combined with their specially developed operator interface "LDplus" (Lyo-Display), live up to the challenges of safe and successful freeze drying day after day.

Innovative technology gives results
• Compact, high-performance laboratory equipment requires little space
• The building-block principle allows procedural and capacity expansion with a far-reaching variety of accessories
• Ice condenser chambers with high-quality stainless steel inner condenser coils
• Easily accessible ice condenser, simple facilities for defrosting, cleaning and decontamination (GMP/GLP requirements), drain valve included
• Visual control of condenser icing during drying
• Low performance losses and no condensation of water due to high-quality thermal insulation of the ice condenser chamber
• Drying chamber located directly above the ice condenser provides high sublimation performance and reduced drying time
• Sample freezing in the ice condenser chamber from -25°C (single-stage compressors) to -50°C (double-stage compressors) and gentle freeze drying
• Digitally displayed ice condenser temperature and indirect determining of the product temperature corresponding to the vapour pressure curve above ice, vacuum control for optimization of process times (option)
• Data interface RS 232 optional, e.g. for process documentation software LyoLog LL-1

Technical Information

Rotational-Vacuum-Concentrator RVC 2-18

Compact benchtop system, especially for concentration of DNA/RNA, proteins.

"System-package" with diaphragm-pump or connection to existing pump systems.

Rotor capacities:

• 90 x 0.5/0.75 ml
• 72 x 1.5/2.2 ml
• 18 x 4 - 7 ml
• 12 x 10 -15 ml
• 6 x 15 - 18 ml

Rotational-Vacuum-Concentrator ALPHA-RVC "IR"

Universal benchtop system for "high-speed" evaporation (e.g. Combinatorial Chemistry, High-Throughput-Screening), energy-input by infrared heating, for low- and high-boiling solvents, with vacuum control (1000 ... 0.1 mbar).

Connection to cooling-traps (4 litres,-50°C, -80°C or -105°C).

Rotor capacities:

• 240 x 1.5/2.2 ml
• 48 x 4 - 7 ml
• 36 x 10 -15 ml
• 10 x 50 ml
• 4 x 250 ml
• 6 x Microtiter-plates

Rotational-Vacuum-Concentrator ALPHA-RVC

High capacity benchtop system for concentration of substances with low-boiling solvents, with vacuum measurement and -control.

Connection to diaphragm-pumps, also in combination with cooling-traps (2 or 4 litres, -50°C, -80°C or -105°C).

Rotor capacities:

• · 240 x 1.5/2.2 ml
• · 48 x 4 - 7 ml
• · 36 x 10 -15 ml
• · 10 x 50 ml
• · 4 x 250 ml
• · 6 x Microtiter-plates

Rotational-Vacuum-Concentrator BETA-RVC

Special system for fast sample concentration, efficient infrared-heating and temperature control system (e.g. Combinatorial Chemistry, High-Throughput-Screening), for low- and high-boiling solvents, with vacuum control (1000 ... 0.1 mbar), programmer for 10 temperature-/pressure-ramps.

Connection to cooling-traps (4 litres,-50°C or -80°C).

Rotor capacities:

• · 20 x 1.5/2.2 ml
• · 30 x 50 ml
• · 12 x 150 ml
• · 10 x 320 ml
• · 4 x 250 ml
• · 24 x Microtiter-plates or 8 xDeepwell-plates
• · customized rotors on request

Special feature: The BETA-RVC can be integrated within automized processes.

Vacuum Concentrators - Christ

RVC 2-25 CDplus

• the mid-size concentrator: all-purpose table-top concentrator for the routine concentration of larger sample volumes
• rotor capacity 108 x 1.5/2.2 ml tubes up to 2 x microtiter plates
• stainless steel/glass compound cover with window (corrosion proof, e.g. for DCM/ACN)
• chemical resistant diaphragm pumps (2m3/h suction, 7 mbar final vacuum), standard, vacuum system with lower vacuum and vacuum controls, optional
• optional combination with cooling traps (2 or 4 Liters) for increased evaporation

Technical Information

Vacuum Concentrators - Christ

RVC 2-18 CD

• the mini concentrator: inexpensive and compact table-top concentrator suited for smaller sample volumes
• special DNA and HCL systems available
• chemical resistant diaphragm pumps (2m3/h suction capacity, 7 mbar final vacuum), standard, vacuum system with lower vacuum and vacuum controls, optional
• normally used without cooling traps

System solutions for special processes

The complete RVC 2-18 DNA system is designed for the concentration of DNA / RNA in ethanol/methanol / water solutions in up to 72 samples of 1.5 ml / 2.2 ml tubes. A special RVC 2-18 HCl (see photo left) version exists for material containing in hydrocloric acid. For other applications the systems can be configured according to need – please contact us for assistance.

Technical Information

Vacuum Concentrators - Christ

RVC 2-33 CDplus

• The maxi concentrator: a multipurpose system for large sample volumes with practically all types of solvents
• rotor capacity 216 x 1.5/2.2 ml tubes up to 12 x microtiter plates or 4 x deep-well plates
• stainless steel/glass compound cover with window (corrosion proof)
• high-performance drive system, 1.550 min-1 (500xg f. micro tubes) prevents bumping and foaming (e.g. with DCM)
• practically tolerant to imbalance (> 50 g)
• c hemical resistant diaphragm pumps (2m3/h suction, 9 mbar final vacuum), standard, vacuum system with lower vacuum and vacuum controls, optional, chemical hybridpump for solvents with high boiling points (e.g. DMSO)
• recommended use with 4 liter cooling traps due to high volume evaporation (-50°C, -60°C, -90°C or -105°C)
• flexible use as vacuum drying chamber through easy dismantling of rotor shaft

Rotational-Vacuum-Concentrator SpeedDry 2-33IR

Fastest possible Sample Concentration and Drying This newly developed CHRIST-vacuum concentrator SpeedDry 2-33IR is particularly suitable for gentle concentration of DNA/RNA, proteins and similar analysis preparations at the workplace. The versatile rotor program provides a high sample capacity, e.g. from 216 x 1.5 ml reaction vials up to 12 x microtiter-plates or 4 x deep-well-plates. In addition, large sample volumes, e.g. 100-ml-tubes that are used for the screening of active substances can be quickly concentrated thanks to the efficient energy supply with halogen IR lamps.

The innovative drive system with its powerful external rotor motor, the contact-free, central rotary coupler, and direct power transmission all guarantee safe operation even in the event of high unbalance. The user-friendly CDplus control unit provides a quick overview of the current operating parameters. Heating temperatures between +30 °C and +80 °C and, of course, also the entire pressure range of the vacuum pump can be selected either manually or controlled by a program, also freeze drying possible.

Technical Information

Christ Rotational-Vacuum-Concentrators ("RVC")