• Avestin high pressure homogeniser from Wolflabs Product update Introduction Avestin B15 high pressure homogeniser from Wolflabs. The Emulsiflex B15 from Avestin is a benchtop high pressure homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The B15 is air/gas powered and requires no electrical power to run. The pump is designed to ensure silent operation. Features and benefits - Capacity of 2-15ml batch. - Minimum sample size 2 ml. - Requires compressed air/gas. - No electric power needed. - Suitable for use in cold room.   Product category Manufacturer Wolf Staff Date Homogenisers - High Pressure Avestin David Suddaby 23-May-2019

• Avestin high pressure homogeniser from Wolflabs Product update Introduction Avestin C5 high pressure homogeniser from Wolflabs. The EmulsiFlex-C5 from Avestin is a benchtop homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The unique EmulsiFlex design ensures that there are no "O"-rings or gaskets in the entire path of the product. This means less risk of contamination, easier cleaning and no leakage. Pressures of up to 30,000psi enable the EmulsiFlex-C5 to process the toughest samples. With a footprint of 43x17x31 cm, and weighing only 25kg, the C5 is compact enough to fit onto the most overcrowded lab bench without compromising power or performance. Features and benefits - Specially designed pump motor pilot valve ensures quiet operation.. - Pneumatically controlled, dynamic homogenizing valve. - Easy disassembly for cleaning or inspection.. - Same valve is used in larger models for scalable performance. - Stringent inlet and outlet temperatures can be controlled with the installation of a cooling coil or heat exchanger. - Suitable for cold room use. Product category Manufacturer Wolf Staff Date Homogenisers - High Pressure Avestin David Suddaby 23-May-2019

• Avestin high pressure homogeniser from Wolflabs Product update Introduction Avestin EmulsiFlex-C3 high pressure homogeniser from Wolflabs. The EmulsiFlex-C3 from Avestin is a benchtop homogeniser ideal for a wide range of applications including nanoparticles, emulsions, liposomes and lysis of even tough cells like E-Coli and yeast. The EmulsiFlex-C3 is electrically powered with a pump designed to ensure quiet operation. The unique Emulsiflex design ensures there are no "O"-rings or gaskets in the entire path of the product. This means less risk of contamination, easier cleaning and no leakage. Features and benefits - Specially designed pump motor pilot valve ensures quiet operation. - Pneumatically controlled, dynamic homogenizing valve. - Easy disassembly for cleaning or inspection. - Same valve is used in larger models for scalable performance. - Constant, low-through capacity of up to 3l/hr, depending on homogenising pressure. - Adjustable homogenizing pressure in the range of 500 - 30,000psi. Product category Manufacturer Wolf Staff Date Homogenisers - High Pressure Avestin David Suddaby 23-May-2019

• Special Offer - Homogenisers - High Pressure Special offers Prices starting at £871 - https://www.wolflabs.co.uk/laboratory-products/homogenisers-high-pressure How to get the best out of your high pressure homogeniser - It is important that the user identifies the homogenising pressure that will achieve lysis of those particular cells, as pressures that are too low may prove ineffective. - Pressure and temperature are directly affected by each other. As a general rule, a 1000psi (69bar) increase in pressure results in a 1°C increase in temperature, which could potentially impact homogenisation results negatively – it is therefore important that pressure is controlled. - Tougher samples generally require higher pressures for processing. In the case of DNA or protein isolation, it is crucial that the optimal homogenising pressure for a particular sample does not induce a temperature high enough to damage sensitive intracellular products. - Temperature increase may therefore induce an increase in oil viscosity and thus emulsion viscosity, so it can directly and significantly alter the quality of the end product, given optimal viscosity of an emulsion is always crucial. Avestin machines can offer that precise temperature control via compatibility with cooling coils, as well as sanitary heat exchangers. - It should be noted that some products require high homogenising pressures for processing; however these pressures can damage sensitive products. For example, proteins may be denatured. A solution to this problem lies in lowering the pressure and increasing the number of passes through the machine. Multi-pass, high-pressure homogenisation has been shown to induce higher microbial inactivation than single-pass high-pressure homogenisation of which may be a preference to some. - In summary, the identification of the optimal parameters when processing samples via high pressure homogenisation, can allow both ease of use and cost-effectiveness. Product category Manufacturer Wolf Staff Date Homogenisers - High Pressure Avestin David Child 04-Oct-2018

• New Avestin high pressure homogenisers from Wolflabs Product update New LiposoFast and EmulsiFlex series of high pressure homogenisers from Avestin have been added.  Product category Manufacturer Wolf Staff Date Homogenisers - High Pressure Avestin David Suddaby 10-Apr-2018

FAQs - Homogenisers – High Pressure

What is high pressure homogenisation?
It is a mechanical process involving the forced passage of a liquid sample through a narrow nozzle/gap at high pressures such as 30,000 psi.

What are common applications of homogenisation?
Cell disruption, emulsions, dispersions, liposomes and particle size reduction.

Why is it the preferred technology?
Homogenisation is scalable and removes the need for the addition of chemicals such as enzymes for lysis.

Are homogenising pressures adjustable?
Yes, between 500-45,000psi on the B15, and 500-30,000psi on the C3 and C5 machines.

How is temperature controlled?
Optional sanitary heat exchangers and cooling coils are available for the Avestin units.

How can you minimise the effects of proteases?
In the case of protein extraction, temperature control may be critical to minimising the effects of proteases. Proteases are released during cell lysis, which may digest some types of intracellular proteins. Keeping the sample cooled slows down this digestion, and in addition, appropriate protease inhibitors may be added to the solution. The sheer speed of the high pressure homogenisation technique offers a distinct advantage over other lysis methods in that the time target proteins are broken down by proteases is very short. In other words, it allows a high yield in a short time.

Why is high pressure homogenisation sometimes the preferred method for cellular disruption?
Since high pressure homogensation can efficiently lyse cells without the aid of solvents and enzymes, the extracted proteins are more likely to be in their natural form compared to other methods. In other words, they are far less likely to alter in shape. High pressure homogenisation is widely the preferred method for cellular disruption, due to greater yield of viable product.